Physiopathologic aspects of germ cell proliferation : genomic and non genomic effects

Previous activities

Identification of the genes involved in the control of germ cell  proliferation and characterization of the molecular mechanisms leading to abnormal expresion should allow a better understanding of germinal insufficiency, testicular or prostatic carcinogenesis and their links with environment.
Group 1 (G.Pointis) studies the connexins (Cx) involved in the control of germ cell proliferation, interactions between these different connexins especially Cx 43 and 33, and their relation with other partners of the junctional plate (ZO-1, cSrc, N-cadherin, REG) and is looking for modification of these relations in germ cell proliferation disturbances with a special interest in traffic troubles described for Cx in most pathological circonstances (cancer, exposition to tumoral promoters).
Group 2 (P.Fénichel) studies the role of estrogen receptors, androgen receptors and apoptotic actors using an experimental model of wild type and genetically modified mice exposed to endocrine disruptors (xenoestrogens and antiandrogens) in order to identify the mechanisms of exaggerated apoptosis associated to hypofertility and exaggerated proliferation associate with malignant cell proliferation.  This study has a particular concern in hormone dependent epigenetic modifications possibly induced in utero and able to induce transcriptional alterations in adult.
All these aspects concern works on cell lines, transgenic models, human collections (testicular biopsies and testicular tumors) obtained through clinical networks. They take also advantage of the insertion in the axis 1 of C3M called cancer, apoptosis, proliferation, and of the support of grants (Inca, ANR, Europe) of industrial partners (Pfizer, Bayer/CropScience and Schering) and will allow medical application and valorisation (prevention, biotest of toxicity, early diagnosis markers, and therapeutical targets).

Last publications

Siddeek B, Lakhdari N, Inoubli L, Paul-Bellon R, Isnard V, Thibault E, Bongain A, Chevallier D, Repetto E, Trabucchi M, Michiels JF, Yzydorczyk C, Simeoni U, Urtizberea M, Mauduit C, Benahmed M. Developmental epigenetic programming of adult germ cell death disease: Polycomb protein EZH2-miR-101 pathway.
Epigenomics. 2016 Nov;8(11):1459-1479.

Borowiec AS, Sion B, Chalmel F, D Rolland A, Lemonnier L, De Clerck T, Bokhobza A, Derouiche S, Dewailly E, Slomianny C, Mauduit C, Benahmed M, Roudbaraki M, Jégou B, Prevarskaya N, Bidaux G. Cold/menthol TRPM8 receptors
initiate the cold-shock response and protect germ cells from cold-shock-induced oxidation. FASEB J. 2016 Sep;30(9):3155-70.

Team Publications