Cytometry

Our facility is equipped with 3 analyzers and two cell sorters. We also provide a technical expertise, theoretical and technical training as well as cell sorting service. The facility is accessible to internal (all C3M teams) and external users (industry & academics). Two engineers (M.Nebout and F.Larbret) are responsible for analytical instrument training, general daily instrument maintenance/troubleshooting, data analysis, experimental design and general flow cytometry consultation.

Equipment :

The flow cytometry facility operates two cell sorters (BD FACSAriaI and Sony) and three cell analyzers (MACSQuant™ Analyzer 10, MACSQuant™ VYB, BD FACSCanto™ II)

CELL SORTERS:

  • BDFacsAriaI 
  • Blue (488nm), Violet(405) and Red (633nm) off set lasers
  • 8 Colours
  • 4 ways cell sorting/ clonal sorting into plates
  • Operator based services
  • Sony SH800Z
  • Blue (488nm), Violet(405), yellow (561nm) and Red (633nm) off set lasers
  • 8 Colours
  • 2 ways cell sorting
  • Non operator based services

CELL ANALYSERS

  • Miltenyi Devices
  1. MACSQuant™ VYB
  • Blue (488nm), Violet(405) and yellow (561nm) off set lasers
  • 8 Colours
  • 96 wells plate reader
  1. MACSQuant™ Analyzer 10
  • Blue (488nm), Violet(405) and red (635nm) off set lasers
  • 8 Colours
  • 96 wells plate reader
  • BDFACSCanto II
  • Blue (488nm), Violet(405) and Red (633nm) off set lasers
  • 8 Colours
  • Carrousel loading tubes

Contacts:

Scientific manager :

Stoyan Ivanov, Stoyan.IVANOV@univ-codeazur.fr

Technical managers : 

Marielle Nebout, Marielle.NEBOUT@univ-coteazur.fr

Frédéric Larbret, Frederic.LARBRET@univ-coteazur.fr