The histology core facility, supported by the Canceropôle PACA, provides state-of-the-art equipments in histology to perform tissues embedding (paraffin/frozen), sectioning (paraffin/frozen) and staining. We also assists in the setting up and optimization of histological approaches for each scientific project, delivering protocols that can be tested by the researchers themselves. Training support is provided. After training, users have free access to the equipments in the core facility like microtomes or cryostats. The facility is open to outside investigators.
STP 120 Spin Tissue Processor
Modular Tissue Embedding Center EC 350
Microtome Microme HM340E
Microtome Leica RM2055
The STP 120 Spin Tissue Processor runs overnight from Monday to Thursday at 6PM. Fixed samples have to be placed in tissue embedding and processing cassettes. Importantly, before adding the cassette in the Spin Tissue Processor, the cassettes that contain tissues have to be kept for at least one hour in 70% Ethanol. The next day, the cassettes have to be transferred to the Modular Tissue Embedding Center EC 350 that dispenses warmed-up paraffin for making the blocks. Each researcher has to do his own blocks.
Fresh or fixed samples have to be incubated with Optimal Cutting Temperature media (Tissue Tech) and freezes to embed the tissue. Solid blocks are kept at -80°C until use. When needed, the blocks are unmounted and paste on metal holders for sectioning. Blocks are trimmed and then sections of required sickness are collected.
Whole Tissue Clearing Processing
Tissues are stained for desired labelling and cleared by sequential incubation with various solvents. For further information contact the facility manager.
All available equipments at the facility can be booked on the C3M reservation website. Training is necessary before working with them. Please send a Training request to the facility manager. In case of question or help request, please send an email to the facility manager.
Facility Manager: Jerome Gilleron
Co-Facility Manager: Sandrine Marchetti
For any information please contact the facility manager: firstname.lastname@example.org