Confocal A1R-FLIM (Nikon)

  • Confocal laser scanning microscope
  • Resonant scanner for fast live imaging
  • Piezo Z controle for fast 3D imaging
  • Photomanipulation techniques
  • Automated acquisition (JOBS)
  • Perfect focus system (PFS)
  • Picoquant FLIM/FCS module

Hybrid-scanner (galvano + resonant): The resonant scanner is capable of taking up to 30 frame per second (512 x 512 pixels) and 420 fps (512 x 32 pixels). It can be combined with the piezo Z control to achive 3D imaging of intercellular fast dynamics. The hybrid scanner head allows simultaneous photomanipulation and ultrafast imaging. It allows acquisition of rapid changes after photomanipulation (FRAP, photoactivation, photoconversion…).

Detectors: 4 detection channels including two GaAsP which provide higher signal with low noise level.

Excitation wavelengths: 405, 488, 561 and 640nm

Objectives: 2x air, 10x air, 20x air, 40x water, 40x oil, 60x oil

Suitable for fixed and live samples, large sample imaging, FCS, deep imaging

Automation: Using the JOBS module, it is possible to program complex automated acquisitions on multiwell plates/slides.

Picoquant module:

Fuorescence Lifetime Imaging Microscopy (FLIM): For FRET detection, label free tissue imaging, Fluorophores discrimination. Picoquant FLIM module incudes a 488 nm pulsed laser and one hybride detector.

FCS: One color FCS and combination of FILM and FCS (FLCS)

 

To come :

More live imaging: Temperature and CO2 environmental chamber

Two color FLIM and FCS + laser ablation: 405 nm pulsed laser and a second hybride detector

Leica DM5500B

  • Suited for immunofluorescence, fixed samples mounted between microscope slide and coverslip
  • Imaging fluorophores from near UV to Far Red
  • Transmission: phase contrast and polarisation

 

This upright microscope is particularly suited for imaging thin fluorescent samples (cell culture, tissue sections < 5µm) mounted between slide and coverslip.

It is equipped with a low noise high resolution digital CCD camera (Hamamatsu ORCA-ER) and a set of objectives ranging from 10x to 100x

Leica AM TIRF

  • Live cell imaging in wide field fluorescence, TIRF and brightfield
  • Environmental chamber (temperature and CO2)
  • Compatibility with many types of samples : Slides, MatTek, Labteck, Multiwell plates
  • Multipositions acquisitions
  • Three fluorescent channels : GFP, YFP, RFP

 

TIRF microscopy: is particularly suited for imaging of membrane processes such as endocytosis, cellular adherence or any structure located nearby the surface of the coverslip.

The microscope is equipped with a scanner that allows automatic setting of the TIRF angle and the adjustment of the required TIRF penetration depth to the various wavelengths.

Thanks to the automatic AOTF control, it is possible to switch quickly between the laser lines at a constant penetration depth. The evanescent field penetrates the specimen with depth going from 70 nm up to 300 nm.

Zeiss Microdissecteur PALM

  • Laser microdissection, laser ablation
  • Color camera (H&E, β-gal assays...)
  • Compatibility with many types of samples : Slides, MatTek, Labteck, Multiwell plates
  • Transmisson: phase contrast and polarisation
  • Fluorescence (see technical caracteristics sheet)

 

Laser microdissection: Select and isolate the material of interest with precision and no contamination.

Microdissection is achived using a 355 nm pulsed laser with the possibility to cut with submicromiter resolution. Cut samples are collected using pressure catapulting technology. A range of objectives (from 5x up to 150x) allows working on large tissue samples down to intracellular level. The cells/tissues of interest can be identified using brightfield or fluorescence imaging. Thick samples up to a 100 µm can be cut. In addition to fixed samples, isolation of live cells is also possible (for analysis or reculivation). Collected material can be subsequently analyzed for DNA, RNA or proteins.

Applications:

  • selectively harvest tissue regions or individual cells
  • selectively isolate fetal cells, sperm cells, down to chromosomes
  • select and isolate individual cells from Cytospin preparations and smears
  • isolate living cells from fresh tissue and cell cultures

Subsequent analysis:

  • Analyze DNA, RNA and proteins
  • Microarrays
  • Recultivate living cells
  • Select efficiently from heterogeneous cell cultures

Zeiss LSM 510 META

  • Imaging fluorophores from UV to Far Red
  • 3D imaging by Z-sectioning
  • Time lapse acquisitions (environmental chamber ; Temperature and CO2)
  • Spectral acquisition (fluorophores unmixing, autofluorescence removal)
  • Tile scan for large field acquisitions
  • Multipositions acquisition

 

 

 

 

Zeiss Axiovert200M_Metamorph

  • Live cell imaging in wide field fluorescence and brightfield
  • Environmental chamber (temperature and CO2)
  • Fast live imaging (down to 10 ms per image)
  • Compatibility with many types of samples : Slides, MatTek, Labteck, Multiwell plates
  • Multiposition timelapse acquisitions
  • Imaging fluorophores from UV to Far Red

 

Equipment:

  • Zeiss Axiovert200M inverted microscope
  • HBO100 autoalignment source
  • Camera Coolsnap HQ2
  • Metamorph
  • Märzhäuzer motorized stage

 

Objectives: 20x air (NA 0.4), 40x air (NA 0.6), 63x air (NA 0.75), 40x oil (NA 1.3), 100x oil (NA 1.3)